- Title
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Intestinal Disorder in Zebrafish Larvae (Danio rerio): The Protective Action of N-Palmitoylethanolamide-oxazoline
- Authors
- Di Paola, D., Natale, S., Iaria, C., Cordaro, M., Crupi, R., Siracusa, R., D'Amico, R., Fusco, R., Impellizzeri, D., Cuzzocrea, S., Spanò, N., Gugliandolo, E., Peritore, A.F.
- Source
- Full text @ Life (Basel)
PEAOXA effect on DSS-induced intestinal alteration. Longitudinal sections of the mid-distal intestinal junction were stained with haematoxylin and eosin (H&E); (A) CTRL, (B) DSS, (C) DSS+PEAOXA 3 mg/L; (D) DSS+PEAOXA 10 mg/L. (E) Mortality rate. PHENOTYPE:
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PEAOXA effect on DSS-induced mucopolysaccharides production. Whole-mount control and DSS-exposed larvae stained with Alcian blue. Longitudinal sections of the mid-distal intestinal junction from control and DSS-exposed larvae, stained with Alcian blue; (A) CTRL, (B) DSS; (C) DSS+PEAOXA 3 mg/L; (D) DSS+PEAOXA 10 mg/L. Increase mucus production (blue staining) (E). Values represent the mean ± SE *** p < 0.001 compared with CTRL at the same time point and # p < 0.05 relative to DSS 0.1% group. PHENOTYPE:
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Effect of PEAOXA on mRNA expression of cytokine genes along the zebrafish intestine in presence (+) or absence (-) of DSS and PEAOXA 3 or 10 mg/L exposure. Expression values are normalised against the expression of β-actin mRNA, which was not significantly different between any of the treatment groups. Values represent the mean ± SE *** p < 0.001 compared with CTRL at the same time point and ### p < 0.001 relative to DSS 0.1% group. |
Effect of PEAOXA on mRNA expression of ER-related genes (hspa5, chop, ire1, xbp1s, and atf6) along the zebrafish intestine presence (+) or absence (-) of DSS and PEAOXA 3 or 10 mg/L exposure. Expression values are normalised against the expression of β-actin mRNA, which was not significantly different between any of the treatment groups. Values represent the mean ± SE *** p < 0.001 compared with CTRL; ### p < 0.001 relative to DSS 0.1% group. |