FIGURE SUMMARY
Title

Intestinal Disorder in Zebrafish Larvae (Danio rerio): The Protective Action of N-Palmitoylethanolamide-oxazoline

Authors
Di Paola, D., Natale, S., Iaria, C., Cordaro, M., Crupi, R., Siracusa, R., D'Amico, R., Fusco, R., Impellizzeri, D., Cuzzocrea, S., Spanò, N., Gugliandolo, E., Peritore, A.F.
Source
Full text @ Life (Basel)

PEAOXA effect on DSS-induced intestinal alteration. Longitudinal sections of the mid-distal intestinal junction were stained with haematoxylin and eosin (H&E); (A) CTRL, (B) DSS, (C) DSS+PEAOXA 3 mg/L; (D) DSS+PEAOXA 10 mg/L. (E) Mortality rate.

PEAOXA effect on DSS-induced mucopolysaccharides production. Whole-mount control and DSS-exposed larvae stained with Alcian blue. Longitudinal sections of the mid-distal intestinal junction from control and DSS-exposed larvae, stained with Alcian blue; (A) CTRL, (B) DSS; (C) DSS+PEAOXA 3 mg/L; (D) DSS+PEAOXA 10 mg/L. Increase mucus production (blue staining) (E). Values represent the mean ± SE *** p < 0.001 compared with CTRL at the same time point and # p < 0.05 relative to DSS 0.1% group.

Effect of PEAOXA on mRNA expression of cytokine genes along the zebrafish intestine in presence (+) or absence (-) of DSS and PEAOXA 3 or 10 mg/L exposure. Expression values are normalised against the expression of β-actin mRNA, which was not significantly different between any of the treatment groups. Values represent the mean ± SE *** p < 0.001 compared with CTRL at the same time point and ### p < 0.001 relative to DSS 0.1% group.

Effect of PEAOXA on mRNA expression of ER-related genes (hspa5, chop, ire1, xbp1s, and atf6) along the zebrafish intestine presence (+) or absence (-) of DSS and PEAOXA 3 or 10 mg/L exposure. Expression values are normalised against the expression of β-actin mRNA, which was not significantly different between any of the treatment groups. Values represent the mean ± SE *** p < 0.001 compared with CTRL; ### p < 0.001 relative to DSS 0.1% group.

Acknowledgments
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