FIGURE SUMMARY
Title

Interferon regulatory factor-7 is required for hair cell development during zebrafish embryogenesis

Authors
Hu, S.Q., Xu, H.M., Qian, F., Chen, C.S., Wang, X., Liu, D., Cheng, L.
Source
Full text @ Dev. Neurobiol.
Fig. 1

Expression of irf7 in zebrafish larvae. irf7 expression was detected with whole‐mount in situ hybridization at 48 hpf (a–c) and 72 hpf (d–f), respectively. The irf7 mRNA was expressed in the otic vesicle and lateral line (indicated by red arrowhead)

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
Fig. 2

irf7 knockdown zebrafish embryos exhibit severe defects in hair cell (HC) development. (a) Phenotypes of control and irf7‐specific morpholino‐injected Tg(Brn3c:GFP) zebrafish larvae at 72 hpf. (b) Fluorescent imaging of green fluorescent protein (GFP)‐labeled HCs indicates the HC clusters on the lateral line of zebrafish larvae at 72 hpf. (c) Quantification of the number of HC clusters in control and irf7‐specific morpholino‐injected Tg(Brn3c:GFP) zebrafish embryos at 72 hpf. (d) Comparison of the phenotypes of crista HCs (blue dotted circles) in control, irf7‐specific morpholino‐injected and irf7‐specific morpholino & mRNA‐coinjected zebrafish embryos. (e) Quantification of the number of crista HCs in control, irf7‐specific morpholino‐injected and irf7‐specific morpholino & mRNA‐coinjected zebrafish embryos. Error bars indicate standard deviation. ***, P < 0.001

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. 3

Apoptotic analysis of hair cells (HCs) in the neuromasts of control and irf7‐knockdown embryos. DAPI, green fluorescent protein (GFP) and TUNEL (TMR red) were used to label the live cell nuclei, HCs and apoptotic HCs, respectively

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. 4

Significant differentially expressed genes (DEGs) regulated by irf7. (a) Number of DEGs upon knockdown of irf7. Among these DEGs, 18 genes are annotated to hair cell (HC) and posterior lateral line (PLL) neuromasts development. (b) Comparison of relative gene expression of the annotated 18 genes between irf7‐morphants and control. (c) atp1b2b mRNA injection rescues crista HC defects induced by irf7‐knockdown. ***, P < 0.001; ****, P < 0.0001

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. 5

Working model of the Irf7 and Atp1b2b in zebrafish hair cells. Irf7, as a transcription factor, can positively regulate the Atp1b2b, which was a component of the Na+, K+‐ATPase
Fig. S2

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. S3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth
Fig. S4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
text only

Unillustrated author statements

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Neurobiol.
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