- Title
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Estrogen directly stimulates LHb expression at the pituitary level during puberty in female zebrafish
- Authors
- Li, G., Tang, H., Chen, Y., Yin, Y., Ogawa, S., Liu, M., Guo, Y., Qi, X., Liu, Y., Parhar, I.S., Liu, X., Lin, H.
- Source
- Full text @ Mol. Cell. Endocrinol.
Gonad histology and real-time PCR analysis of related expression profiles of pituitary reproductive genes in peripubertal female zebrafish. Fish were grouped into PG (n = 7), PV (n = 6) or EV(n = 7) by their ovarian stages (A–C). A, only primary growth (PG) follicles could be found in the fish with body weight of 85.7 mg. B, previtellogenic (PV) stage follicles started to emerge in the fish with body weight of 111.5 mg. C, both early vitellogenic (EV) and PV stage follicles could be found in the fish of body weight of 134.8 mg. The expression profiles of lhb (D), fshb (E), cyp19a1b (F), esr1 (G), esr2a (H) and esr2b (I) in the pituitary were analyzed by real-time PCR. Relative expression was calculated using the comparative threshold (2−ΔΔCt) method. Data were present as mean ± SEM. Different letters above each bar denote significantly different. PG, primary growth; PV, previtellogenic follicle; EV, early vitellogenic follicle. EXPRESSION / LABELING:
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Double labeling in situ hybridization of lhb mRNA and three types of nER. A, D and G: lhb mRNAs were labeled in green; B, E and H: the three types of nERs (esr1, esr2a and esr2b respectively) were labeled in magenta; C, F and I: in each line, lhb mRNA and nER labeling images were superimposed with NIS Elements D3.2; Nikon. lhb mRNA was found to be colocalized with esr1 and esr2a (white arrows) but not esr2b in the pituitary glands of early pubertal female zebrafish. Scale bar: 20 μm. EXPRESSION / LABELING:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. EXPRESSION / LABELING:
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Reprinted from Molecular and Cellular Endocrinology, 461, Li, G., Tang, H., Chen, Y., Yin, Y., Ogawa, S., Liu, M., Guo, Y., Qi, X., Liu, Y., Parhar, I.S., Liu, X., Lin, H., Estrogen directly stimulates LHb expression at the pituitary level during puberty in female zebrafish, 1-11, Copyright (2017) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.