ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

The induction profiles of proteasome subunit genes. Whole-mount in situ hybridization was performed to analyze the induction profiles of psma3, psma5, and psmb7 using 4 dpf nrf2a^fh318 mutant larvae treated with or without 100 μM DEM for 12 h. The arrowheads indicate positive expression in the liver, and asterisks denote the basal expression in the intestine. The numbers in each picture indicate the positive/tested larvae.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

The induction profiles of glucose metabolism-related genes. Whole-mount in situ hybridization was performed to analyze the induction profile of pgd and fbp1a using 4 pdf nrf2a^fh318 mutant larvae treated with or without 100 μM DEM for 6 h. The arrowheads indicate positive expression in the liver and gills, and asterisks denote the basal expression in the intestine. The numbers in each picture indicate the positive expression in the liver/tested larvae.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

The expression of the proteasome subunit genes. (a) The gene expression of the indicated proteasome subunits in 8 h postfertilization (hpf) wild-type embryos injected with or without 100 pg of nrf2a mRNA at the 1-cell stage was analyzed by a real-time qPCR. Total RNA was extracted from 30 embryos for each sample. The expression of each gene was normalized to that of ef1α (means ± SEM), and the value in uninjected control was set to 1. Asterisks denote statistical significance (Control versus nrf2a overexpression, ^∗∗P < 0.01; Student's t-test, n = 6 for each group). (b) and (c) The gene expression of the indicated proteasome subunits in 4 dpf wild-type or nrf2a^fh318/fh318 mutant larvae that were treated (or not treated) with 100 μM DEM for 6 h (b) and 12 h (c) was analyzed by a real-time qPCR. The expression of each gene was normalized to that of ef1α (means ± SEM), and the value in untreated wild-type control was set to 1.

The induction profiles of proteasome subunit genes. Whole-mount in situ hybridization was performed to analyze the induction profiles of psma3, psma5, and psmb7 using 4 dpf nrf2a^fh318 mutant larvae treated with or without 100 μM DEM for 12 h. The arrowheads indicate positive expression in the liver, and asterisks denote the basal expression in the intestine. The numbers in each picture indicate the positive/tested larvae.

The expression of glucose metabolism-related genes. (a) The upregulated gene lineups from the three microarray experiments were compared. The data of DEM- or tBHQ-treated zebrafish larvae are from Nakajima et al. [19] and Hahn et al. [20], respectively. Numbers in parentheses and in the Venn diagrams denote the numbers of genes which belong to each category. The names of 27 overlapping genes were displayed. (b) The gene expression of the indicated enzymes related to glucose metabolism in 8 hpf wild-type embryos injected with or without 100 pg of nrf2a mRNA at the 1-cell stage was analyzed by a real-time qPCR. Asterisks denote statistical significance (Control versus nrf2a overexpression, ^∗P < 0.05; Student's t-test, n = 6 for each group). (c) The gene expression of the indicated enzymes related to glucose metabolism in 4 dpf wild-type or nrf2a^fh318/fh318 mutant larvae treated with or without 100 μM DEM for 6 h was analyzed by a real-time qPCR. Asterisks and hash marks denote statistical significance (DEM+ versus DEM−, ^∗P < 0.05 and ^∗∗P < 0.01; wild-type versus nrf2a^fh318/fh318, ^#P < 0.05; Student's t-test, n = 6 for each group).

The induction profiles of glucose metabolism-related genes. Whole-mount in situ hybridization was performed to analyze the induction profile of pgd and fbp1a using 4 pdf nrf2a^fh318 mutant larvae treated with or without 100 μM DEM for 6 h. The arrowheads indicate positive expression in the liver and gills, and asterisks denote the basal expression in the intestine. The numbers in each picture indicate the positive expression in the liver/tested larvae.

The expression of other candidate genes for zebrafish Nrf2 targets. (a) The expression of the indicated genes in 8 hpf wild-type embryos injected with or without 100 pg of nrf2a mRNA at the 1-cell stage was analyzed by a real-time qPCR. Asterisks denote statistical significance (Control versus nrf2a overexpression, ^∗P < 0.05 and ^∗∗P < 0.01; Student's t-test, n = 6 for each group). (b) The expression of the indicated genes in 4 dpf wild-type or nrf2a^fh318/fh318 mutant larvae treated with or without 100 μM DEM for 6 h was analyzed by a real-time qPCR. Asterisks and hash marks denote statistical significance (DEM+ versus DEM−, ^∗P < 0.05 and ^∗∗P < 0.01; wild-type versus nrf2a^fh318/fh318, ^#P < 0.05 and ^##P < 0.01; Student's t-test, n = 6 for each group).