- Title
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Cilia-Associated Genes Play Differing Roles in Aminoglycoside-Induced Hair Cell Death in Zebrafish
- Authors
- Stawicki, T.M., Hernandez, L., Esterberg, R., Linbo, T., Owens, K.N., Shah, A.N., Thapa, N., Roberts, B., Moens, C.B., Rubel, E.W., Raible, D.W.
- Source
- Full text @ G3 (Bethesda)
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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Intraflagellar transport (IFT) but not transition zone mutants show defects in kinocilia morphology. Neuromasts of 5 dpf zebrafish larvae stained with acetylated tubulin to label kinocilia. (A) Mutations in the IFT genes dync2h1, wdr35, ift88, and traf3ip cause a lack of kinocilia in cells. A mutation in the ciliary axoneme gene arl13b causes a shortening, but not an absence, of kinocilia. (B) Kinocilia morphology appears unchanged by mutations in the transition zone genes cc2d2a, mks1, and cep290. Images to the far left are of representative wild-type sibling. No differences were observed among siblings of the different mutants. Scale bar = 10 µm. Mut, mutant; WT Sib, wild-type sibling. PHENOTYPE:
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Early developmental defects are not seen in the hair cells of zebrafish cilia mutants. (A) Phalloidin labeling of the stereocilia of 5 dpf zebrafish hair cells show no gross morphological defects in dync2h1 or cc2d2a mutants. Scale bar = 10 µm. (B) Earlier in development, at 3 dpf, dync2h1 mutants have grossly normal kinocilia. Scale bar = 10 µm. (C) Decreases in hair cell number in the absence of neomycin in dync2h1 mutants is not seen until 5 dpf. Data are shown as mean ± SD. n = 10 fish per group. **** P < 0.00001 by two-way ANOVA and Šídák multiple comparisons test. dpf, d post-fertilization; Mut, mutant; WT Sib, wild-type sibling. |
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
|