PUBLICATION

Mitotic patterns in the migrating lateral line cells of zebrafish embryos

Authors
Laguerre, L., Ghysen, A., and Dambly-Chaudière, C.
ID
ZDB-PUB-090407-6
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(5): 1042-1051 (Journal)
Registered Authors
Dambly-Chaudière, Christine, Ghysen, Alain
Keywords
neuromast, cell proliferation, primordium, cell migration, quiescence, determinate lineage
MeSH Terms
  • Animals
  • Body Patterning*
  • Cell Movement*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Lateral Line System/cytology*
  • Lateral Line System/embryology*
  • Mitosis*
  • Zebrafish/embryology*
  • Zebrafish/physiology
PubMed
19334282 Full text @ Dev. Dyn.
Abstract
The sense organs of the posterior lateral line system (neuromasts) are formed by a migrating primordium. In zebrafish, the primordium comprises approximately 100 cells at the onset of migration, and has deposited approximately 300 cells by the end of the process. Here, we report localized phases of mitotic activity and of mitotic quiescence within the migrating primordium. Quiescence in the leading region seems associated to the formation of a new prospective neuromast, whereas quiescence in the trailing region follows a wave of mitoses that synchronize trailing cells in G0/G1 phase, anticipating neuromast differentiation. Manipulating the size of the primordium does not lead to changes in the rate of cell proliferation. We also show that two mitoses often take place nearly synchronously in adjacent cells, suggestive of a determinate lineage. We conclude that proliferation in the migrating primordium follows a stereotyped pattern that closely anticipates the normal development of the system.
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