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Fig. 7 Retinoic acid regulates krt18a.1, but not krt8, expression in the eye during early embryogenesis. (A–F) Treatment of Casper zebrafish embryos at 24 and 27 hpf with DEAB and RA, respectively, followed by wholemount colorimetric in situ analysis shows decreased krt18a.1, but not krt8, expression in the developing eye in response to pharmacological insult. (G) Quantification of these effects, as measured in the anterior chamber (orange dashed box) and hyaloid (yellow dashed box) regions of the eyes of 48-hpf zebrafish, further shows that compared with the DMSO vehicle control, treatment with either DEAB or ATRA significantly decreased krt18a.1 expression, while that of krt8 was not affected. *, p-value < 0.05.