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Figure Caption
Fig. 5 tjp1a-RFP embryos show localization of RFP to regions of cell–cell contact, and immunolabeling demonstrates that the RFP is colocalized with Tjp1a. (a–c) Confocal images of RFP in the tjp1a-RFP line in the trunk at 40 × with transmitted light in (b). Larva was 3 dpf. (d–f) Immunolabeling with anti-dsRed antibodies display a junctional pattern at 1 dpf at 63 × . (e) A similar pattern of labeling was observed with anti-Tjp1a antibodies. (f) Overlap of images in (d) and (e). NC, notochord; DA, dorsal aorta; CV, caudal vein, and the arrow indicates a segmental blood vessel.
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