ZFIN Image: Pomreinke et al., 2024, Fig. 4

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Figure Caption

Fig. 4

Expression of hemangioblast/endothelial and erythrocyte maturation markers in nampt-a loss-of-function conditions. nampt-a^+/+ (+ / +) and nampt-a^t10pm/t10pm (-/-) embryos were injected with nampt-a morpholino (MO). Embryos were assayed at 1 dpf for the expression of the hemangioblast/endothelial marker kdrl (A) and the erythrocyte markers gata1a (B) and klf1 (C) by qRT-PCR. Fold change was calculated using the ∆∆C_t method [26]. Each point represents one biological sample of ten embryos pooled for RNA extraction. Each colored line represents the average from separate experimental trial days with the black line representing the average from all trials. For statistical analysis, the Kruskal–Wallis non-parametric test was applied followed by Dunn’s multiple comparison test to determine p-values. D Effect size (η²) for the Kruskal–Wallis test to compare each group

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Acknowledgments

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