ZFIN Image: Ma et al., 2024, Fig. 4

Image

Figure Caption

Fig. 4 PSEN1 is modulated by METTL3-mediated m⁶A RNA methylation.

A The protein level of PSEN1 in BMSCs, HEPM cells and DPSCs with METTL3-knockdown and control groups. B The relative expression of PSEN1 detected at the RNA level after treatment with DAA in at various concentrations (0 μmol, 600 μmol and 700 μmol). C, D Potential m⁶A sites in full-length PSEN1 gene predicted using SRAMP. E The wild-type or mutant m⁶A consensus sequence fused with the firefly luciferase reporter. F–H The transcription levels of wild-type and mutant PSEN1 in BMSCs, HEPM cells and DPSCs. I–K The m⁶A methylation level of the PSEN1 at specific modification site (m⁶A1585) and control site (A1579) using SELECT in control and METTL3 knockdown cells. Results were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 or ***P < 0.001 indicates a significant difference between the groups.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.