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Fig. 8 Normal initiation of gene expression in the chamber myocardium of smarcc1a mutants. Whole-mount in situ hybridization compares expression patterns of nppa (A–D) and bmp10 (E, F) expression at 36 (A, B) or 48 (C–F) hpf. Dorsal views, ventricle to the top. (A, B) The initial expression of nppa is similar in wild-type and smarcc1a mutant hearts at 36 hpf. (C, D) At 48 hpf, wild-type expression of nppa is found in the chamber myocardium but not in the AVC (arrowhead). In smarcc1a mutants, elevated expression is seen throughout the heart, including in the AVC (arrowhead). (E, F) In both wild-type and smarcc1a mutant hearts at 48 hpf, bmp10 expression is found in the cardiac chambers (arrow), with a clear gap in expression at the AVC.