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Fig. 5 Cardiac chamber identity is established normally in the smarcc1a mutant heart tube. (A–L) Whole-mount in situ hybridization compares expression of myl7 in all cardiomyocytes (A, D, G, J) with expression of vmhc in ventricular cardiomyocytes (B, E, H, K) and amhc in atrial cardiomyocytes (C, F, I, L). (A–F) Dorsal views at 30 hpf, ventricle to the top. (G–L) Frontal views at 48 hpf, ventricle to the top. vmhc and amhc expression are found in complementary domains corresponding to ventricular and atrial identities in both wild-type embryos and smarcc1a mutants within the linear heart tube (A–F) and as development proceeds (G–L).