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Fig. 5 RT-qPCR analysis after 72 hpe exposure of PFOA and alternatives to zebrafish. (a) mRNA expression levels of representative target genes downstream of PPARα; (b) mRNA expression levels of representative target genes downstream of SREBP; (c) mRNA expression levels of sterol transport-related factors; (d) represents the correlation analysis between RNA-seq gene expression level and RT-qPCR, and the adjusted R2 was Gen-X 0.86, PFOA 0.87, HFPO-TA 0.85. n = 5, 30 zebrafish per group; * P < 0.05 compared with control group; data are expressed as mean ± SE.

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