Fig. 5

Larval Chchd10 compensates for loss of Chchd2 expression but not vice versa and is present in both a lower and higher molecular weight complex in zebrafish at 5 dpf. (ai) Representative immunoblot of Chchd10 protein level in chchd2^−/− larvae aged 5 dpf. (aii) Immunoblot quantification (5 dpf) showing increased Chchd10 expression compared to wild type in chchd2^−/− larvae (t-test, p = .011). Actin was used as loading control. (aiii) Unchanged transcript levels of chchd10 in 5 dpf chchd2^−/− larvae (t-test, p = .30 relative to actin). (bi) Representative immunoblot of Chchd2 protein expression in chchd10^−/− larvae aged 5 dpf. (bii) Immunoblot quantification (5 dpf) showing reduced Chchd2 expression levels in chchd10^−/− larvae compared to wild type larvae (t-test, p = .001). Actin was used as loading control. (biii) Unchanged transcript levels of chchd2 in 5 dpf chchd10^−/− larvae (t-test, p = .55, relative to actin). (c) Second dimensional PAGE analysis using a Chchd10 antibody revealed a high molecular weight complex, close to 140 kDa, in both wild type and chchd2^−/− larvae. Mt-Co1 and Sdhb are used as molecular weight markers. Sample sizes (n) are noted in parentheses. Significant differences from wild type are represented by either a single asterisk (p < .05) or double asterisk (p < .01).