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Figure 5

Fold change of gene expression in cep290 and bbs2 mutant retinas compared to wild-type animals. qRT-PCR of selected genes involved in inflammatory signaling and apoptosis processes in 6 mpf mutants. Gene expression was normalized to 18S reference gene expression and plotted relative to expression in wild-type siblings. Data are plotted as the mean ± S.D. Three technical replicates were conducted on each of four distinct biological samples of RNA. At least 2 retinas were pooled for each biological sample. *p < 0.05 by way of two-way Mann Whitney tests.

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Acknowledgments
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