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Figure 4
ROS mediated stress is induced in livers of zebrafish fed a high-fat diet and liver-specific cytokine induced embryos. (A–D) Cell Rox Deep Red staining used to visualize ROS-mediated stress in the livers of control, HFD-fed, Li-PIC3, and Li-PIC+HFD larvae. (E) Quantification of Cell Rox intensity demonstrated an increase in only Li-PIC3+HFD embryos as compared to Tg(fabp10:cre) controls, while the comparison to controls trended towards significance (p = 0.776). (F) Quantification of ldh1a mRNA levels by qPCR (G) Quantification of ggt mRNA levels by qPCR. Both ldh1a and ggt are elevated in the combined Li-PIC3+HFD condition. Statistical analysis was by one-way ANOVA, n = 4–5 per condition; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.