ZFIN Image: Lu et al., 2021, Fig 3

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Figure Caption

Fig 3 Notch1a and Notch3 receptors participate in the differentiation of agr2⁺ epidermal mucous cells. (A). notch1a or X-Su(H)-ANK-overexpressing embryos display significant increases in agr2⁺ EMC numbers in the trunks and yolks compared to LacZ-overexpressing embryos at 30 or 24 hpf. (B). Similar agr2⁺ EMC numbers in the trunks and yolks were detected in notch1a homozygous mutants or notch3 morphants compared to sibling wild-type or wild-type embryos at 24 hpf. Substantial reductions in agr2⁺ EMC numbers in the trunks and yolks are detected in notch1a homozygous mutants injected with 0.4 or 0.6 ng notch3 UTRMO compared to sibling wild types injected with the same amount of notch3 UTRMO at 24 hpf. Arrows, EMCs. Scale bars, 100 μm. Mean ± SEM. Student’s t-test. *p<0.05; **p<0.01; ***p<0.001; ns, not significant. Underlying data are available in S1 Data.

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Acknowledgments

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