Fig. 3

Fig. 3

A Proportion of hematopoietic cells in whole kidney marrow of 6-month-old asxl1^+/+, asxl1^−/− (n = 9 and n = 10, respectively; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). Mye P myeloid progenitors, Mye mature myeloid cells, Ery erythrocytes, Lym lymphocytes, Neu neutrophils, Mac macrophages, Eos eosinophils. May–Grünwald–Giemsa staining of whole kidney marrow from 6-month-old asxl1^+/+ and asxl1^−/− (scale bar, 20 μm; black boxes show enlarged details of neutrophils; red arrows, neutrophils). B May–Grünwald–Giemsa staining of neutrophils from kidney marrow. Neutrophils were quantified by morphology. Neutrophils were collected from ten adult fish of each genotype asxl1^+/+ or asxl1^−/−. After staining, 500 cells were randomly chosen for further calculation. This process was repeated three times for statistical tests (scale bar, 20 μm; black boxes show enlarged details of neutrophils; red arrows, neutrophils; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, error bars, mean ± SD). C qRT-PCR comparing expression of myeloid markers in sorted neutrophils from asxl1^+/+ and asxl1^−/−. Neutrophils were collected from ten adult fish of each genotype asxl1^+/+ or asxl1^−/−. qPCR was performed with three technical replicates (two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, error bars, mean ± SD) (color figure online).