ZFIN Image: Fittipaldi et al., 2021, Figure 4

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Figure Caption

Figure 4

smyd3 knockdown promotes mesendodermal fate in zebrafish. (A) Expression of the mesendodermal markers, gata6, gata5 and mespaa, was evaluated by ISH in smyd3 morphants at 50% of epiboly, in comparison to standard control embryos. Embryos are shown in dorsal view. (B) ISH of the ectodermal marker otx3 was performed in morphants and standard control embryos at 50% epiboly stage. (C) The endodermal marker sox17 was examined at 75% epiboly and shown in dorsal view. (D) ISH of dorsal mesodermal markers gsc, chrd and of ventral mesodermal marker gata2 was performed at 50% epiboly and shown in dorsal view. Scale bar: 250 μm.

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Acknowledgments

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