ZFIN Image: Zhao et al., 2021, Fig. 2

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Figure Caption

Fig. 2 MicroRNA-1 knockout affects neural crest cell apoptosis during the migration and differentiation periods of embryonic development. In situ hybridization of zebrafish at 24 hpf with (A) foxd3, (B) msxb, (C) snai1b, (D) tfap2a, (E) dlx3b and (F) ngn1 probes. (G) Whole mount immunostaining of zebrafish at 24 hpf with PHH3 (red) and DAPI (blue). (H) Quantitative analysis of immunostaining. (I) TUNEL staining at 24 hpf with TUNEL (red) and DAPI (blue). (J) Quantitative analysis of TUNEL staining. Data are presented as the mean ± standard deviation (n=5). *P<0.05 vs. WT. WT, wild-type; foxd3, forkhead box D3; msxb, muscle segment homeobox-B; snai1b, snail homolog 1b; tfap2a, transcription factor AP2A; dlx3b, distal-less 3; ngn1, neurog1; PHH3, phospho-histone H3; hpf, hours post fertilization; ns, not significant.

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Acknowledgments

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