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Fig. 5 Fig. 5. MZcdx1b−/− mutant exhibited significant reduction in foxa2-and sox17-expressing endodermal precursors. RNA in situ hybridization was conducted on wild-type (WT) and MZcdx1b−/− mutant embryos at 85% epiboly using foxa2 (A) or sox17 (B) RNA probes. Quantification of respective numbers of foxa2-or sox17-expressing endodermal precursors among wild types and MZcdx1b−/− mutants is shown. Scale bar represents 100 μm. Student’s t-test was conducted. ∗∗∗, p < 0.001.
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Reprinted from Developmental Biology, 470, Wu, C.S., Lu, Y.F., Liu, Y.H., Huang, C.J., Hwang, S.L., Zebrafish Cdx1b modulates epithalamic asymmetry by regulating ndr2 and lft1 expression, 21-36, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.