ZFIN Image: Nagashima et al., 2019, Figure 7.

Image

Figure Caption

Figure 7.

The mdka^mi5001 mutant upregulates “core” transcriptional regulators following photoreceptor death. A–C, qPCR for dedifferentiation markers, ascl1a, stat3, and lin28, at 30 and 36 hpl. Both WT and mdka^mi5001 upregulate ascl1a (A; WT: 30 hpl, p < 0.0001, 36 hpl, p < 0.0001; mdka^mi5001: 30 hpl, p = 0.0004, 36 hpl, p = 0.006, p = 0.0066 relative to WT; F-ratio = 37.5606), lin28 (B; WT: 30 hpl, p < 0.0001, 36 hpl, p = 0.0095; mdka^mi5001: 30 hpl, p < 0.0001, 36 hpl, p = 0.0004; F-ratio = 32.9337), and stat3 (C; WT: 30 hpl, p = 0.0048, 36 hpl, p = 0.0016; mdka^mi5001: 30 hpl, p = 0.0004, 36 hpl, p = 0.0016), following lesion. ANOVA with post hoc Tukey, relative to unlesioned. D, E, Western blot for Stat3 in WT and mdka^mi5001 retinas at 1 dpl. WT: p = 0.0002, mdka^mi5001: p = 0.0004, ANOVA with post hoc Tukey; F-ratio = 40.8763. F, Immunocytochemistry for phosphorylated Stat3 (pStat3) in the WT and mdka^mi5001 mutant. In unlesioned retina, immunosignal for pStat3 is not detected in the inner nuclear layer. Following photoreceptor lesion, Müller glia in the inner nuclear layer upregulate pStat3 in WT, whereas mdka^mi5001 mutants have reduced phosphorylation of Stat3. Scale bar, 30 μm. inl, Inner nuclear layer; ipl, inner plexiform layer. *p < 0.01.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.