Fig. 8.

Fig. 8.

Antioxidant treatment partially rescues hair cell numbers in the inner ear and the lateral line of ak2^hg14 mutants. (A,B) Average number of mature hair cells in the inner ear cristae (top) and anterior macula (bottom) of 3 dpf (A) or in the inner ear cristae of 4 dpf (B) ak2^hg14 embryos treated with different concentrations of GSH (100-300 µM). Results are shown as mean±s.e.m. (one-way ANOVA, followed by Tukey's post hoc test; P<0.001). (C) Summary of the percentage of rescue induced by the different GSH treatments on the inner ear cristae and anterior macula at 3 and 4 dpf. (D) Quantitative analysis of the rescue induced by GSH treatments on the expression of alkaline phosphatase in PLL neuromasts of 4 and 5 dpf ak2^hg14 and control embryos. Numbers above bars indicate the total amount of embryos used for the analysis. a, anterior; m, medial; p, posterior; UNTR, untreated. A one-way ANOVA (followed by Tukey's post hoc test; P<0.001) was used to compare groups of fish treated differently.