ZFIN Image: Tomecka et al., 2019, Fig. 2.

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Figure Caption

Fig. 2.

Non-union fractures in a zebrafish OI model. (A-D) Brightfield images at low (A,B) and high (C,D) magnification of adult fins at 3 months post fertilisation, showing thickening and dark foci in frf mutants (B,D) compared with WT (A,C). (E,F) Magnified images of WT (E) and frf (F) foci stained with Alizarin Red demonstrates bone calluses at these sites, indicating fractures. (G,H) Brightfield and fluorescent images of induced fractures in WT (G) and frf (H) at the indicated dpc. Bone was alternately stained with calcein (green) or Alizarin Red (red) to visualise callus growth. (I) A proportion of induced fractures in frf were non-union; n=12 per genotype. (J) The gap size following fracture was not reduced compared with WT over time; n=12 per point. **P<0.01, ***P<0.001; ANOVA with Sidak post test. Scale bars: in B, 2 mm for A,B; in D and F, 200 µm for C-F; in H, 100 µm for G,H.

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Phenotype details

Acknowledgments

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