|
Fig. 2 S1
Z yap1-/- mutants only exhibit mild lymphatic defects in the trunk and MZ yap1-/- mutants form facial lymphatics.
( A) Trunk lymphatics of sibling and Z yap1ncv101-/- mutant at 5 dpf. The vasculature is shown in red [ Tg(kdrl:mCherry)], venous and lymphatics in green [ Tg(dab2b:EGFP)]. Scale bar: 50 μm. ( B) Heads of siblings and MZ yap1ncv101-/- mutants showing facial lymphatics including the lateral facial lymphatic (LFL), medial facial lymphatic (MFL), otolithic lymphatic vessel (OLV), branchial arch lymphatics (LAA); and developing lymphatic loop (LL). Scale bar: 50 μm. ( C) Percentage of thoracic duct (TD) segments formed across 6 somites at 5 dpf in siblings (98.17 ± 1.83, n = 18) and Z yap1ncv101-/- mutants (77.54 ± 10.14, n = 13; p=0.0269). ( D) Score of facial lymphatics in siblings (LL: 0.75 ± 0.08, n = 10) and MZ yap1ncv101-/- mutants (LL: 0.67 ± 0.11, n = 6; p=0.55 (ns)). Each point represents the vessel score for one embryo. Matrix scores for LFL, MFL and OFL between sibling and MZ yap1ncv101-/- are identical, thus not significantly different.