ZFIN Image: Turner et al., 2019, Fig. 5

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Figure Caption

Fig. 5

slbp^ty77e mutant cells fail to transition from proliferation to differentiation.

(A,A’) DNA content of 48 hpf wildtype and slbp^ty77e embryos as assessed by flow cytometry. (B,B’, C,C’) Transverse sections through 48hpf wildtype (B,C) and slbp^ty77emutant (B’,C’) eyes immunostained with anti-BrdU (B,B’) and anti-pH3 antibodies (C,C’). (D,D’) Series of projections of confocal images extracted from live time-lapse movies of tg(EF1α:mAG-zGem(1/100))^rw0410h; tg(EF1α: mKO2-zCdt(1/90))^rw0405b transgenic wildtype (D) and slbp^ty77e mutant (D’) embryos showing cell cycle progression in cells of the forming somites. Green cells are proliferative (S, G2, M) whereas red cells are differentiating somite cells. Scale bar: (B-C’) 50μm; (D-D’) 300μm.

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Acknowledgments

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