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Fig. 3 Pparα deficiency reduced neuronal precursors. A–D: In situ hybridization demonstrating the down‐regulation of neurog1, ascl1a, ascl1b, and neurod1 expression in embryos treated with GW6471, pparaa ΔLBD, pparab ΔLBD, or coinjection of pparaa ΔLBD and pparab ΔLBD (A,B), which was confirmed through quantitative real‐time polymerase chain reaction (C,D). * P < 0.05; **P < 0.01. Staging of the embryos is shown in the bottom left corner of each panel in A and B.
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