Fig. 4

Aqp0a is required for normal lens optics. Representiative lenses dissected from WT, aqp0a^−/−, aqp0b^−/−, or double mutant zebrafish of SL >23 mm imaged through their optical axis (equatorial orientation). Lenses imaged under brightfield (BF; [A]) and darkfield (DF; [B]) illumination reveal a clear lens for WT and aqp0b^−/− mutants, while aqp0a^−/− mutants have mild refractive changes between the nucleus and lens periphery. Double mutant lenses have a severe nuclear cataract with cortical refractive problems. (C) Optical properties of lenses were tested by focusing through the lens onto micrometer grids, which revealed poor optical properties of the aqp0a^−/− and double mutant lenses.