Fig. 6
Fig. 6
zTreg Cells Express Tissue-Specific Pro-regenerative Factors
(A?C) qRT-PCR analysis of growth factor expression in injured tissues (mean ± SEM, n = 6).
(D?F) qRT-PCR analysis of secreted factors in purified zTreg cells (mean ± SD; ?p < 0.05, ??p < 0.01). Gene expression is shown relative to the levels in kidney-derived zTreg cells.
(G) In situ hybridization using RNAscope and immunofluorescence against TagRFP in damaged foxp3a:RFP tissues. Arrows indicate zTreg cells expressing mRNAs of ntf3 (left), nrg1(middle), or igf1(right). Single confocal sections are shown.
(H) Semi-qRT-PCR analysis of zTreg cells purified from injured tissues and uninjured kidneys.
(I?K) Experimental interventions involving NTF3 (I), NRG1 (J), or IGF1 (K) treatments. i.p., intraperitoneal injection; i.v., intravitreal injection.
(L?N) Quantification of proliferating neural progenitor cells (L), cardiomyocytes (M), and Müller glia (N) (n = 5?8). Representative images are shown in Figures S4B, S4F, and S4G.
?p < 0.05, ??p < 0.01, Mann-Whitney U test (A?C and L?N) and Student's t test (D?F). Scale bars, 20 ?m. See also Figure S4.
Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell