ZFIN Image: Takada et al., 2017, Fig. 3

Image

Figure Caption

Fig. 3

Characterization of ?vesselless?. (A). Schematic description of the ?vesselless? model and the experimental strategy. Green, vECs, endocardium and hematopoietic cells; red, cardiomyocytes. (B). Specific absence of hematovascular cells (i.e. vECs, endocardium, hematopoietic cells) in ?vesselless?. Green, vECs and endocardium; red, cardiomyocytes. Scale bars: 1?mm (top); 1?mm (middle); 50?�m (bottom). (C). qRT-PCR analysis of pan-hypoxia indicator genes in ?vesselless?. *P<0.05; ***P<0.001; n.s., not significant. Student?s t-test. n=8 (WT siblings), 8 (?vesselless?). (D). GO enrichment analysis of ?vesselless? (blue) and ?heartless? (red). The top 44 GO terms are shown. Highlighted are neural/sensory (green) and sterol (yellow)-related GOs. (E). Volcano plot representing differentially expressed genes in ?vesselless?. Shown are genes that are specifically affected in ?heartless? (red) and in ?vesselless? (blue). n=2. (F). qRT-PCR analysis of cholesterol biosynthesis genes. **P<0.01; ***P<0.001. n.s., not significant. Student?s t-test. n=8 (WT siblings), 7 (?vesselless? at 2?dpf), 8 (?vesselless? at 4.5?dpf). (G). Summary of differentially expressed genes in ?vesselless?.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open