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Fig. 8
Knockdown of sept7b affects glucose homeostasis.
A) The amount of whole body glucose is significantly increased in sept7b knockdown larva compared to control MO-injected larvae at 5 dpf. DMSO in buffer served as a negative control. (B) qRT-PCR indicates that 40 mM exogenous glucose treatment for 24 h increases insa expression in wild type and control MO-injected zebrafish larvae at 5 dpf. insa expression is downregulated in sept7b knockdown larvae compared to the controls. Treatment of sept7b knockdown larvae with exogenous glucose increases insa expression, but the level remains clearly lower compared to wild type and control MO-treated larvae with or without exogenous glucose treatment. (C) qRT-PCR indicates that 40 mM exogenous glucose treatment for 24 h downregulates pck1 expression in wild type and control MO-injected zebrafish larvae at 5 dpf. pck1 expression is upregulated in sept7b knockdown larvae compared to the controls. Treatment of sept7b knockdown larvae with exogenous glucose decreases pck1 expression, but the level remains clearly higher compared to wild type and control MO-treated larvae with or without exogenous glucose treatment. This indicates defective regulation of glucose homeostasis in sept7b knockdown larvae. In (B,C), insa and pck1 were normalized to β-actin. Error bars represent mean ± SEM. *p ≤ 0.05; **p ≤ 0.005; ***p ≤ 0.0005.
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