ZFIN Image: Sun et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Alkbh4 associates and co-localizes with Atrn. (A) Whole-mount in situ hybridization results of putative Alkbh4 binding partners, including atrn, ranbp10 and mpi. Only the expression pattern of atrn is similar to that of alkbh4 at 24 hpf. (B) Flag-ALKBH4 was co-expressed with HA-Atrn in HEK293T cells and their interaction was examined by immunoprecipitation. HA-Atrn was immunoprecipitated with anti-HA antibody and the precipitates were examined by western blotting for the presence of ALKBH4 with anti-Flag antibody. TCL, total cell lysate. (C) Co-localization of ALKBH4-GFP with HA-Atrn in zebrafish embryonic cells. HA-Atrn plasmid DNA and ALKBH4-GFP mRNA were injected into one cell at 4-cell stage of the embryos. Embryos were double stained with corresponding antibodies at 75% epiboly stage. Scale bars: 100 μm in (A); 10 μm in (C).

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Expression / Labeling details

Acknowledgments

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