ZFIN Image: Dash et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Shh expression is missing from endoderm of sept7b knockdown embryos.

(A) Lateral view of 24 hpf zebrafish embryo injected with the control MO showing expression of sonic hedgehog (shh) in the endoderm (arrowhead) and notochord (arrow). (B) Endodermal expression of shh (arrowhead) is missing from sept7b knockdown embryos whereas expression in notochord is present (arrow). (C) Co-injection of sept7b cRNA with sept7b TBMO partially rescues the expression of shh in the endoderm (arrowhead). (D) Notch receptor expression is downregulated in sept7b knockdown larvae. The expression of notch1a and notch1b mRNAs is significantly downregulated in sept7b knockdown larvae compared to control MO-injected larvae. Co-injection of sept7b cRNA with sept7b TBMO partially rescues downregulation of notch1a and notch1b. The expression of notch1a and notch1b were normalized to actin. (E) The expression of ascl1b mRNA is increased at 12 hpf and 5 dpf in sept7b TBMO-injected larvae compared to control MO-injected larvae. Scale bar: (A–C) (50 μm). Error bars represent mean ± SEM. *p ≤ 0.05; **p ≤ 0.005; ***p ≤ 0.0005.

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Acknowledgments

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