Fig. 7
Accumulation of mutant p53 protein in tumors of p53M214K/M214K fish. Adult p53M214K/M214K zebrafish that were observed to have developed tumors in the eye and trunk were sacrificed, fixed and embedded in paraffin for histological analysis with the p53-5.1 hybridoma supernatant. The sections were counterstained with hematoxylin and imaged under brightfield. (a) Normal eye from a p53M214K/M214K fish ( × 10 magnification). (b) Tumor in the eye of a p53M214K/M214K fish ( × 10 magnification). (c) Tumor in the eye of a p53M214K/M214K fish ( × 40 magnification). (d) Tumor in the trunk of a p53M214K/M214K fish ( × 40 magnification). P, pigmentation of eye lens.