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Fig. 4
Expression patterns of dhps and eif5a in zebrafish.
(a) Pathway illustrating the connection between the polyamine biosynthetic pathway, eIF5A, and DHS. Wholemount in situ hybridization for (b) dhps and (c) eif5a at 48 hpf and 72 hpf. At 72 hpf, higher magnification reveals expression of (d) dhps and (e) eif5a in the liver, intestine and pancreas. (f) Fluorescent in situ hybridization of eif5a in Tg(neurod1:gfp) transgenic fish at 72 hpf confirms expression in endoderm-derived organs, including swim bladder (SWB), intestine, liver, and pancreas.
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