Fig. 2

Fig. 2

Up-regulation of p53 in the retinae and brain of klf8 morphants can be rescued by co-injection with klf8 mRNA. (A–E, G–J) Dorsal views of embryos. Enhanced p53 expression was detected in embryos injected with klf8-MO1^atg (C) or klf8-MO2^atg (D), or co-injected with klf8-MO^AC and klf8-MO^DO (E), but not in WT (A) or klf8-4mmMO1-injected (B) embryos at 24 hpf. (F) Percentage of klf8 morphants and control embryos displaying enhanced p53 expression. The increase in p53 expression in klf8 morphants (H) as compared to WT (G) was rescued by co-injection with different amounts of klf8 mRNA (J), but not with LacZ mRNA (I). (K) Percentage of klf8 morphants co-injected with klf8 mRNA or LacZ mRNA displaying enhanced p53 expression. Significance was determined using Student′s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate standard errors.