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Fig. 5
Knockdown of hif2-alpha damages liver cell proliferation.
WT (Tg(lfabp:EGFP)) embryos (A) and hif2-alpha ATG-MO-injected embryos (B) were examined for liver cell proliferation using an anti-pH3 antibody at 4 dpf. Cell proliferation in hif2-alpha ATG-MO-injected embryos was reduced compared with WT embryos. (C) Quantification of pH3-positive cells in the liver (n = 11, p<0.05). (D) The EGFP-positive cells were counted by FACS. Quantification of pH3-positive cells in the trunk (E) and tail (F) (n = 4, p<0.05).
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