Fig. 1
Validation of microarray results by in situ hybridization using whole mount in situ hybridization on 5 dpa regenerating fins.
(A) hapln1a expression is reduced in sof b123 compared with WT (B). alf dty86 expression is variable (C,D). (E,F) In situ hybridization on a WT 5 dpa cryosection reveals compartment specific expression of hapln1a, mainly in the blastema (b), in the mesenchyme (m), and skeletal precursor cells (*). The thick arrow identifies the basal layer of the epidermis, which underlies the epidermis (e). The thin arrow identifies lepidotrichia and the arrowhead identifies the actinotrichia. The amputation plane is indicated in panels A-D. Scale bar in E and F is 50 μm.