ZFIN Image: Walsh et al., 2011, Fig. S2

Image

Figure Caption

Fig. S2 Segmental patterning and facial branchiomotor (FBM) neuron differentiation is normal in nhsl1b mutants. (A-D) RNA in situ hybridization with hindbrain markers for rhombomere (r)4 (hoxb1a, blue in A,B); r3 and r5 (krox20; red in C,D); r7 and posterior hindbrain (hoxd4; blue in C,D); and otic vesicles (pax2b, blue in C,D). All of these genes are expressed normally in nhsl1b^fh131 mutants (B,D). (E-J) RNA in situ hybridization with motor neuron genes that are required to specify the migratory phenotype: tbx20 (E,F), phox2a (G,H) and tag-1 (I,J). Although FBM neurons fail to migrate caudally in nhsl1b^fh131 mutants, they nevertheless express these genes, indicating that their failure to migrate is not due to failure to activate a migratory transcriptional program. Scale bar: 50 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development