Fig. 1

Fig. 1 Temporal and spatial expression profiles of gata5, gata6, otx2 and prdm1a. (A) Quantitative expression profiles of gata5, gata6, otx2 and prdm1a determined by using Q-PCR. The X-axis represents RNA molecules per embryo defined by Q-PCR. RNA was isolated from embryos at different time points, and the absolute molecular numbers per embryo are shown as a purple line for gata5, a blue line for gata6, a red line for otx2, and a green line for prdm1a. The experiments were conducted in triplicate, and the standard deviation is shown as a bar extending to both sides of the mean. (B–E) In situ hybridization of gata5, gata6, otx2 and prdm1a in wild-type embryos at 5 hpf. gata5 and gata6 are expressed in the mesendoderm and the YSL at 5 hpf. otx2 is expressed in the blastoderm at 5 hpf, and prdm1a is expressed in the blastoderm and ectoderm. Arrow indicates the location of mesendoderm lineage at the margin of blastoderm. (F–K) Co-localization of otx2, gata5 and gata6 at 5 hpf double in situ hybridization for gata6 and otx2 (F, G, H) and gata5 and gata6 (I, J, K) in wild-type embryos at 5 hpf. (F) gata6 mRNA is indicated by green fluorescence. (G) otx2 mRNA is shown by red fluorescence. (H) Merged image of F and G, indicating the co-localization of gata6 and otx2 in the mesendoderm. (I) gata5 mRNA is indicated by green fluorescence. (J) gata6 mRNA is shown by red fluorescence. (K) Merged image of I and J indicating the co-localization of gata5 and gata6 in the mesendoderm. Arrow indicates the location of mesendoderm lineage at the margin of the blastoderm.