ZFIN Image: Bhat et al., 2013, Fig. 7

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Figure Caption

Fig. 7 Misexpression of tfap2a rescues PPE and NC in Bmp-blocked embryos. Expression of PPE markers six4.1 (A) and dlx3b (B) at 10 hpf, NC marker foxd3 at 12 hpf (C), neural plate marker krox20 at 12 hpf (D), competence factors foxi1 (E) and gata3 (F) at 10 hpf, epidermal marker p63 at 10 hpf (G) and Bmp-feedback inhibitor sizzled at 10 hpf (H) in control embryos, embryos treated with 200 μM DM from 4 hpf, or hs:tfap2a/+transgenic embryos treated with 200 μM DM from 4 hpf and heat shocked at 6 hpf. Images show lateral views with dorsal to the right and anterior up. Insets in (A) show ventral views of corresponding specimens. Misexpression of tfap2a in DM-treated embryos rescues PPE and NC markers while causing slight disruption in stripes of krox20, but does not restore expression of other competence factors, p63 or sizzled.

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Acknowledgments

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Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.