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Fig. 10 Activation of the UPR pathway in the sec13sq198 mutant. (A) Quantitative RT-PCR analysis of UPR-related genes bip, chop, grp94, atf6, ire1a and perk in WT and the mutant, respectively. The relative expression level of the genes was shown in fold change as normalized to the housekeeping gene, elf1a. (B) WISH analysis of chop expression in WT and mutant (mu) embryos at 5 dpf. (C) Western blot analysis of Bip, Chop, phosphorylated eIF2a (p-eIF2a) and total eIF2a (eIF2a) in WT amd mutant (mu). β-Actin was used as the loading control.
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Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.