ZFIN Image: Niu et al., 2012, Fig. S14

Image

Figure Caption

Fig. S14 Analysis of sec31b expression patterns. (A) Semi-quantitative RT-PCR analysis of sec31b and sec31b transcripts at the stages of 6 hpf, 12 hpf, 1 dpf, 2 dpf and 3 dpf. First strand cDNA (reverse transcription, RT) was synthesized from total RNA by MMLV reverse transcriptase (Invitrogen) using oligo-dT primers and then used as the template in PCR. PCR was run at 28 cycles of 94oC (30 second), 55oC (30 second), 72oC (20 second). For analyzing sec31a: forward primer: AgCCgTgTCTTCTggAgggTgTT, reverse primer: ggTgCgggAggAgTgAAgTgTTg, For analyzing sec31b: forward primer: ATgAAgCgCgTgTgAAgTTTTTgA, reverse primer: TACggggATCTggAAgggCTgTTT, (B) WISH analysis of sec31b expression at the stages of 6 hpf, 12 hpf, 1 dpf, 2 dpf and 3 dpf. RT product from 1 dpf was used as the template to clone sec31b using primer pair ACgTgAATTCCTCCgCCCCAgCAACAACAACC (forward) and ACgTTCTAgACCCCgAgCAgATgAAgCACAgACA (reverse). PCR product was digested with EcoRI and Xbal1 and then cloned to the pCS2+ vector for generating DIG-labeled WISH riboprobe.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.