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Fig. 1 spaw and lefty1 expression phenotypes. (A-F′′)Time course of single color, double in situ hybridizations for spaw and lefty1 from 10-18 somites in posterior (A-F), mid-LPM (A′-F′), left lateral (A′-F′) and anterior LPM (A′′-F′′) views. (A-A′′) False-colored images of zebrafish embryos from B-B′′ depicting the domains of spaw and lefty1. In situ hybridizations of spaw or lefty1 alone confirm the reported phenotypes. Arrows, boundary of detectable spaw expression; brackets, ectopic spaw across the midline; arrowheads, lefty1 in the diencephalon; asterisks, lefty1 in the cardiac mesoderm. L, left; R, right.