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Fig. 3 Brain neurons of AO127 mutants contain lysosomes engorged with rRNA. Brain transverse sections from embryos 5 d after fertilization. EM revealed abundant electron-dense vesicles in the neurons of AO127 mutants (some of these are indicated by red arrowheads; Inset, electron-dense material is bounded by a limiting membrane). (Scale bars: 5 μm). In situ hybridization revealed accumulation of rRNA aggregates (white arrows) in the cytoplasm of AO127 mutant neurons. (Scale bars: 10 μm.)
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