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Fig. 2 Colocalization of zfPGRN-A and znp1 immunoreactivity in Primary motor neurons at 27 hpf. Lateral views (anterior- left; dorsal- top) of whole-mount embryos labelled with zfPGRN-A (A, D) and znp1 mAb (B, E) at 27 hpf. Embryos show zfPGRN-A expression in the developing eye, tectum and hindbrain (A); Spinal Cord, Somite boundaries and motor neurons (D); znp1 expression in the eye, hindbrain, Spinal Cord and motor neurons (B and E). Merged images show co-localization of zfPGRN-A and znp1 (C, F). Abbreviations: T, Tectum; E, Eye; MHB, Mid-Brain Hindbrain Boundary; SB, Somite boundary; MN, Motoneurons.; SC, Spinal cord. Images were captured at 20 × magnification. Scale Bar = 50 μm.