Fig. 5
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Fig. 5 Hindbrain development in val mutants. All embryos are val homozygous mutants. A: wnt1 expression at 27 hours postfertilization (hpf). B: dlA expression at 30 hpf. C: Two-color in situ hybridization, showing wnt1 (black) and dlA (Fast Red fluorescence) on the left side of the hindbrain at 30 hpf. D: erm expression at 30 hpf. The position of the otic vesicles (ov) is indicated. E: Commissural neurons (cn) labeled with zn8 antibody at 30 hpf. The number and positions of commissural axons is highly variable in the posterior hindbrain. Note that the right side of the r5/6 region has only a single commissure (c), which is not in register with the two commissures on the left. F: Reticulospinal neurons (rsn) labeled with anti-acetylated tubulin at 24 hpf. G: Radial glia labeled with zrf1-4 antibodies at 30 hpf. All images show dorsal views with anterior to the top except for G, which shows a lateral view with anterior to the left. Numbers indicate corresponding rhombomeres. Scale bar in G = 50 μm in C,G, 70 μm in E, 90 μm in F, 120 μm in A,B,D.