ZFIN Image: Veldman et al., 2010, Fig. 10

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Figure Caption

Fig. 10 Regeneration-dependent induction of KLF6a and 7a is necessary for tuba1a and p27 gene induction following optic nerve injury. The optic nerve of adult tuba1a:GFP transgenic fish was lesioned and KLF6a/ KLF7a targeting MO or a control MO was applied to the optic nerve stump as described in Experimental methods. 3 days later retinas were harvested and RNA purified for use in RT-PCR reactions. Shown is an ethidium bromide stained gel showing induction of GFP, tuba1a, p27 and c-jun following optic nerve injury. GAPDH is used as a loading control. Note that KLF6a and 7a knockdown (KLF6/7 MO) caused a significant reduction in GFP, tuba1a and p27 gene induction following optic nerve injury.

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Acknowledgments

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Reprinted from Molecular and cellular neurosciences, 43(4), Veldman, M.B., Bemben, M.A., and Goldman, D., Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish, 370-383, Copyright (2010) with permission from Elsevier. Full text @ Mol. Cell Neurosci.