ZFIN Image: Jia et al., 2009, Fig. 8

Image

Figure Caption

Fig. 8 Smad2/3 activities cooperate with Fgf and Wnt signals in neural induction. (A, F and F′) Uninjected wildtype (WT) embryo. (B–E, G–J and G′–J′) Embryos were injected with indicated mRNA species or morpholino at the following doses: 300 pg dnfgfr1 mRNA; 200 pg dnsmad3b mRNA; and 20 ng β-cat2-MO2. (A–E) Lateral views of embryos showed sox2 expression at the shield stage, with dorsal to the right. (F–J and F′–J′) Lateral (F–J) or dorsal (F′–J′) views showed otx2 and hoxb1b expression at late gastrulation stages, with the anterior to the top. (K, L) Statistical data for the percentage of embryos showing altered expression of sox2 (K) and otx2/hoxb1b (L). (M) Quantitative RT-PCR results showing the relative levels of sox2 transcripts at the shield stage after injections with different mRNA species.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 333(2), Jia, S., Wu, D., Xing, C., and Meng, A., Smad2/3 activities are required for induction and patterning of the neuroectoderm in zebrafish, 273-284, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.