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Figure Caption
Fig. 5 Overexpression of human Pak1 produces embryonic abnormalities in zebrafish that are contingent upon nuclear import of Pak1.
(A) Images of zebrafish embryos at 4 dpf. Embryos were injected with either Pak1 WT-mRNA or one of the mRNA of the Pak1 nuclear import mutants (80 ng/μL). (B) Quantitative analysis of differential zebrafish body axis malformation at 4 dpf in embryos injected with either Pak1 WT-mRNA or one of the mRNA of the Pak1 nuclear import mutants. (C) Immunoblot analysis of lysates from zebrafish injected with either human Pak1-WT, NLSmut, or LC8mut mRNA.
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